PIT-1 Immunostain, Technical Component Only
Use
Pit-1, also known as POU1F1, is a transcription factor involved in the development of the anterior pituitary and is useful in the classification of pituitary adenomas. Expression of Pit-1 is observed in somatotropic hormone-producing tumors such as those producing prolactin, growth hormone, or thyroid-stimulating hormone.
Special Instructions
This test includes only the technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Any immunohistochemistry (IHC)/in situ hybridization (ISH) stain performed as part of the PATHC will be performed at the reviewing pathologist's discretion at an additional charge. Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry. Best practices for storage of positively charged slides include minimizing time slides are stored after being unpackaged, limiting exposure to high humidity and heat, and minimizing exposure to plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Ambient temperature preferred.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides.