Powassan Virus, RNA, Molecular Detection, PCR, Random, Urine
Use
Rapid testing for Powassan virus RNA (lineage 1 and lineage 2) using random urine specimens. This test acts as an adjunctive measure to serology for the detection of early Powassan virus infection, especially during the first few days after symptom onset. It facilitates a timely diagnosis that can help in discontinuing empiric antibiotics and providing prognostic information for patients and families.
Special Instructions
The assay should not be used for screening asymptomatic individuals and is intended only for those with signs and symptoms of Powassan virus disease. For non-electronic orders, complete the Microbiology Test Request Form (T244) and send with the specimen.
Limitations
The sensitivity of the assay is dependent upon the time of illness onset when the specimen is collected. RT-PCR testing is most useful within the first seven days of illness onset. A negative test result does not exclude Powassan virus infection; results should be used in conjunction with clinical findings and serologic test results. The test detects both viable and nonviable virus, and its performance depends on the viral load in the specimen.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 55144-0
- 55144-0
- 77202-0
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
1 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
1. Collect random urine in a sterile container. 2. Label specimen as urine.
Causes for Rejection
Heat inactivated specimen; urine containing preservatives
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |