Powassan Virus, RNA, Molecular Detection, PCR, Serum
Use
The test is useful for rapid testing of Powassan virus RNA (lineage 1 and lineage 2) using serum specimens. It serves as an adjunctive test to serology for detecting early Powassan virus infection, particularly during the first few days after symptom onset. It is specifically recommended for patients who show signs and symptoms of Powassan virus disease, and not for screening asymptomatic individuals. The assay complements serologic testing in diagnosing early infection as both provide essential information for optimal patient care.
Special Instructions
The test should be used during the first 7 days after symptom onset. It is advised to order this test in conjunction with POWV / Powassan Virus, IgM, Enzyme-Linked Immunosorbent Assay, Serum. Specimen handling is critical: centrifuge and aliquot serum into a sterile container within 2 hours of collection. Serum specimens not aliquoted will be rejected. A Microbiology Test Request Form (T244) is needed if not ordering electronically.
Limitations
The sensitivity of the assay depends on the timing of specimen collection relative to the onset of symptoms. It's most effective within the first few days of symptom onset. A negative test does not completely rule out infection, necessitating corroboration with clinical findings and serologic test results. The assay detects both viable and nonviable virus. RNA detection is optimal in serum or blood, but it remains positive in urine for longer. Specificity is high despite the presence of related flaviviruses, with no noted cross-reactions.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 55144-0
- 55144-0
- 77202-0
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
Within 2 hours of collection centrifuge and aliquot serum into a sterile container. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.
Causes for Rejection
Gross hemolysis, Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |