Powassan Virus, RNA, Molecular Detection, PCR, Spinal Fluid
Use
Rapid testing for Powassan virus RNA (lineage 1 and lineage 2) using cerebrospinal fluid specimens. This test is an adjunctive tool to serology for detecting early Powassan virus infection, particularly useful within the first few days after symptom onset. It should not be used for screening asymptomatic individuals, but for patients exhibiting signs and symptoms of Powassan virus disease.
Special Instructions
Not provided.
Limitations
This assay is most useful when used within the first few days of symptom onset. A negative test does not exclude infection with Powassan virus, as the sensitivity decreases after 7 days of illness. This assay detects both viable and nonviable virus, and the performance depends on the viral load present in the specimen, which may not correlate with cell culture results. Improper specimen handling or delayed processing may affect test accuracy.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
Powassan Virus
Microorganism
LOINC Codes
- 34457-2 - POWV RNA CSF Ql NAA+probe
- 34457-2 - POWV RNA CSF Ql NAA+probe
- 77202-0 - Laboratory comment Report
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.3 mL
Container
Sterile vial
Collection Instructions
Send specimen from collection vial 2. Do not centrifuge or heat inactivate.
Causes for Rejection
Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |