Protein, Total, Spinal Fluid
Use
The test for total protein in cerebrospinal fluid (CSF) is useful in detecting disruptions of the blood-brain barrier or intrathecal synthesis of immunoglobulins. Elevated levels of CSF protein are associated with conditions that compromise the integrity of the capillary endothelial barrier, such as meningitis, brain tumors, and cerebral infarction. These conditions lead to an increase in the total protein concentration in CSF due to plasma infiltration or neurological disease processes. The test is particularly useful in diagnosing and monitoring conditions like multiple sclerosis, encephalomyelitis, and various forms of meningitis.
Special Instructions
Not provided.
Limitations
Increased CSF total protein is sensitive for detecting blood-brain barrier disruptions but is not specific for distinguishing between various causes. Hemolysis can falsely elevate protein levels, requiring interpretation with caution, particularly in cases involving traumatic lumbar puncture or CNS hemorrhagic processes. Presence of hemoglobin or blood in CSF can invalidate results. Positive bias from contrast media, such as Iopamidol, Iohexol, and Metrizamide, should be considered.
FDA ApprovedNew York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
Total Protein
Analyte
LOINC Codes
- 2880-3 - Prot CSF-mCnc
- 2880-3 - Prot CSF-mCnc
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.25 mL
Container
Sterile vial
Collection Instructions
Centrifuge specimen to remove any cellular material.
Causes for Rejection
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 72 hours |
| Frozen | 180 days |