Rabies Antibody Endpoint
Use
RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which measures the ability of rabies-specific antibodies to neutralize the rabies virus and prevent it from infecting cells. The antibodies are called rabies virus neutralizing antibodies (RVNA). Quantitative results assist in determining rabies vaccination effectiveness, with a result of 0.5 IU/mL or higher considered an acceptable response according to World Health Organization guidelines.
Special Instructions
Collection date is required for specimen submission. The test is typically performed on Monday, Wednesday, and Thursday.
Limitations
The test requires standard laboratory conditions to ensure accurate results. Specimen degradation or contamination can affect titers, and improper storage may lead to false negatives or unusable samples. Failing to follow the specified collection and handling instructions may result in specimen rejection.
New York Approved
Methodology
Other (Serum Neutralization Fluorescent Antibody)
Biomarkers
LOINC Codes
- 6524-3
- 6524-3
Result Turnaround Time
16-30 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red top (preferred) or serum gel (acceptable)
Collection Instructions
Centrifuge and aliquot 1 mL of serum into a plastic vial. Send refrigerated. Collection date is required.
Storage Instructions
Refrigerate the sample. If freezing, ensure it remains frozen until analysis.
Causes for Rejection
Gross hemolysis, gross lipemia, bacterial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 60 days |