Rapid Plasma Reagin (RPR) with Reflex to Treponema pallidum Particle Agglutination, Serum
Use
The Rapid Plasma Reagin (RPR) test with reflex to Treponema pallidum Particle Agglutination (TP-PA) is primarily used for diagnosing syphilis. Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a systemic infection with periods of latency. Due to its systemic nature, serologic techniques are crucial for diagnosis and follow-up. This test is part of the reverse syphilis screening algorithm. If the initial treponemal antibody assay is positive, it reflexes to RPR testing. Positive RPR results suggest active or recent infection, while negative results reflex to the TP-PA test for confirmation.
Special Instructions
Not provided.
Limitations
Biological false-positive reactions can occur with cardiolipin-type antigens, which are seen in certain diseases like infectious mononucleosis, leprosy, malaria, lupus erythematosus, and others. Pregnancy, certain autoimmune diseases, or narcotic addictions might cause false-positive results. Other treponemal diseases can also lead to false positives. These limitations highlight the need for confirmatory testing in the case of positive results.
FDA ApprovedNew York Approved
Methodology
Other
Biomarkers
Treponema pallidum
Microorganism
LOINC Codes
- 20507-0 - RPR Ser Ql
- 20507-0 - RPR Ser Ql
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.3 mL
Minimum Volume
Not provided
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 14 days |