Relative B-Cell Subset Analysis Percentage, Blood
Use
This test is used for screening humoral or combined immunodeficiencies such as common variable immunodeficiency and hyper IgM syndrome. It helps assess B-cell subset distribution and reconstitution after hematopoietic cell or bone marrow transplant or after recovery following B-cell-depleting immunotherapy.
Special Instructions
Not provided.
Limitations
This test is not indicated for evaluating lymphoproliferative disorders such as leukemia, lymphoma, or multiple myeloma. It should not be used for monitoring B-cell counts in patients undergoing B-cell-depleting therapies; use CD20B for that purpose.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 90416-9 - Lymph subset + B-cell phenotype Pnl Bld
- 8117-4 - CD19 Cells NFr Bld
- 8119-0 - CD20 Cells NFr Bld
- 89358-6 - CD27 Cells/Cells.CD19 NFr Bld
- 89352-9 - CD27+IgD+IgM+/CD19 cells NFr Bld
- 89350-3 - CD27+IgD-IgM-/CD19 cells NFr Bld
- 89348-7 - CD27+IgD-IgM+/CD19 cells NFr Bld
- 89346-1 - IgM cells/Cells.CD19 NFr Bld
- 89344-6 - CD38+IgM- cells/Cells.CD19 NFr Bld
- 89341-2 - CD38+IgM+ cells/Cells.CD19 NFr Bld
- 89356-0 - CD21 Cells/Cells.CD19 NFr Bld
- 89355-2 - CD21- cells/Cells.CD19 NFr Bld
- 69048-7 - Immunologist review
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL (>14 years); 4 mL (<=14 years)
Minimum Volume
5 mL (>14 years); 3 mL (<=14 years)
Container
Lavender top (EDTA)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot. Label specimen as blood for RBCS.
Patient Preparation
For serial monitoring, collect specimens at the same time of day.
Storage Instructions
Ship specimens refrigerated and ensure they arrive within 24 hours of collection.
Causes for Rejection
Gross hemolysis; Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 48 hours |