Riboflavin (Vitamin B2), Plasma
Use
This test is useful for evaluating individuals who present signs of ariboflavinosis, a condition caused by a dietary deficiency of riboflavin (vitamin B2). Ariboflavinosis is characterized by symptoms such as sore throat, cheilosis, angular stomatitis, glossitis, corneal vascularization, dyssebacia, and normocytic, normochromic anemia. It can also affect the conversion of vitamin B6 to its coenzyme and the conversion of tryptophan to niacin. Moreover, subtle deficiencies in riboflavin might lead to negative health consequences and are related to rare inborn errors of metabolism involving riboflavin transporters.
Special Instructions
Ship specimen in an amber vial to protect from light. Fasting is required for at least 12 hours prior to specimen collection; infants should have their specimen collected before the next feeding. The test runs on Monday, Wednesday, and Friday, and the report is available within 2 to 5 days.
Limitations
Test results may be affected by non-fasting status and dietary supplementation of vitamin B2, which can lead to elevated plasma vitamin B2 concentrations. Thus, the accuracy of the test may be compromised under these conditions. Additionally, improper specimen handling or selection and interfering substances could also cause diagnostic confusion.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 2924-9
- 2924-9
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
0.5 mL
Container
Amber vial
Collection Instructions
Collect specimen in a green top (sodium or lithium heparin) tube. Alternatively, use a light-green top (sodium or lithium heparin plasma gel) tube. Centrifuge and aliquot plasma into an amber vial within 2 hours of collection.
Patient Preparation
Fasting: 12 hours, required; Infants should have specimen collected before next feeding
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 28 days |
| Frozen | 28 days |