Sardine (Pilchard), IgE, Serum
Use
This test is useful for establishing a diagnosis of an allergy to sardine (pilchard) and for defining the allergen responsible for eliciting signs and symptoms. It also helps in identifying allergens responsible for allergic response and/or anaphylactic episode, confirming sensitization prior to beginning immunotherapy, and investigating the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens. However, it is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists or in patients for whom the medical management does not depend upon identification of allergen specificity.
Special Instructions
For a listing of allergens available for testing, see the Allergens - Immunoglobulin E (IgE) Antibodies document. If not ordering electronically, complete, print, and send an Allergen Test Request form (T236) with the specimen.
Limitations
Testing for IgE antibodies may result in false positives in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases. Some individuals with clinically insignificant sensitivity to allergens may yield measurable levels of IgE antibodies; therefore, test results must be interpreted in the clinical context.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 30989-8
- 30989-8
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens requested
Minimum Volume
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Container
Preferred: Serum gel; Acceptable: Red top; Submission Container/Tube: Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis: OK; Gross lipemia: OK
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |