Sex Chromosome Determination, FISH, Tissue
Use
This test is useful for identifying the sex chromosome complement in paraffin-embedded tissues, which can help determine the sex of cells in tissue samples. It is particularly helpful in post-transplant contexts to identify opposite sex-donor cells and to resolve cases of suspected sample mix-up. The test uses a commercially available enumeration strategy probe set for the X centromere (DXZ1) and Y centromere (DYZ3).
Special Instructions
Not provided.
Limitations
The test is developed with an analyte specific reagent and is not cleared or approved by the FDA. It is best used as an adjunct to clinical and pathologic information. Non-formalin fixatives may not be successful for FISH assays, although non-formalin-fixed samples will not be rejected. Decalcified paraffin-embedded tissues are generally unsuccessful for FISH analysis and may lead to test cancellation.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Unknown CNV
Copy Number RegionUnknown CNV
Copy Number Region
LOINC Codes
- 87436-2 - Chr X + Y aneup Bld/T FISH
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 87436-2 - Chr X + Y aneup Bld/T FISH
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or slides
Collection Instructions
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Alternative fixation methods may be acceptable with specification. For slides, submit four consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and one H and E-stained slide.
Storage Instructions
Ambient (preferred) or refrigerated
Causes for Rejection
All specimens will be evaluated for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Preferred |