Sezary Diagnostic Flow Cytometry, Blood
Use
Sezary syndrome (SS) and mycosis fungoides are distinct T-cell lymphoproliferative disorders. This test is useful for identifying immunophenotypically aberrant T-cell populations with restricted TRBC expression in peripheral blood, thus estimating circulating tumor burden in cutaneous T-cell lymphomas. Detection of neoplastic CD4-positive T cells in peripheral blood is essential for SS diagnosis. Flow cytometry is used to estimate the number of Sezary cells, aiding in CTCL disease staging and therapy response assessment.
Special Instructions
This test is not indicated for monitoring patients with a confirmed diagnosis of Sezary syndrome. For monitoring, order SZMON / Sezary Monitoring Flow Cytometry, Blood. If not ordering electronically, complete and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Limitations
Correlation with clinical features is necessary for diagnosing Sezary syndrome. This analysis describes a cell population with an aberrant phenotype, but its clinical significance might be uncertain without additional clinical correlation.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 101118-8
- 50398-7
- 30954-2
- 22635-7
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
6 mL
Minimum Volume
1 mL
Container
Preferred: Yellow top (ACD solution A or B); Acceptable: Lavender top (EDTA), green top (sodium heparin)
Collection Instructions
Send the whole blood specimen in the original tube. Do not aliquot. Label specimen as blood.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 days |
| Refrigerated | 4 days |