SMAD4 Immunostain, Technical Component Only
Use
SMAD4 (mothers against decapentaplegic homolog 4 drosophila) is a tumor suppressor and transcription factor part of the transforming growth factor beta signaling pathway. Normally expressed within the nucleus of epithelial cells, loss of SMAD4 expression results from inactivation of the _SMAD4_ gene due to sporadic genetic variants. This expression status is useful for diagnosing pancreatic adenocarcinoma.
Special Instructions
Not provided.
Limitations
The charge of glass slides can be influenced by environmental factors, potentially altering staining. Inconsistent staining may occur due to unsuitable glass slides. Best practices include minimizing time slides are stored after being unpackaged and limiting exposure to high humidity, heat, and plastics. Cut paraffin section age affects immunoreactivity, with practices suggesting cutting within 6 weeks.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
SMAD4
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |