Squash, IgE, Serum
Use
The test is used for establishing a diagnosis of an allergy to squash by detecting IgE antibodies in serum. It helps in defining the allergen responsible for eliciting signs and symptoms and confirms sensitization prior to immunotherapy. Clinically, this test can identify allergens responsible for allergic response and anaphylactic episodes, helping direct patient management and therapy.
Special Instructions
For ordering, complete and submit the Allergen Test Request form if not ordering electronically. Proper specimen collection involves centrifuging and aliquoting serum into a plastic vial. Refrigerated conditions for specimens are preferred for up to 14 days, or they can be frozen for up to 90 days.
Limitations
Testing for IgE antibodies is not recommended for patients previously treated with immunotherapy to ascertain if residual clinical sensitivity exists, nor in cases where medical management does not rely on identifying allergen specificity. False positives can occur in patients with very high serum IgE levels, and results must be interpreted considering clinical conditions.
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 7713-1
- 7713-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens requested
Minimum Volume
0.3 mL for 1 allergen
Container
Preferred: Serum gel; Acceptable: Red top; Submission: Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Storage Instructions
Refrigerated (preferred), 14 days; Frozen, 90 days
Causes for Rejection
Gross hemolysis (OK), Gross lipemia (OK)
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |
