T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies

Casandra

Casandra Test Code MC33083Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID TALAFCPT Codes 88271, 88275, 88291

T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies

Clinical Use

Use

This test is useful for detecting, at diagnosis, recurrent common chromosome abnormalities associated with T-cell acute lymphoblastic leukemia/lymphoma (T-ALL) in adult patients using a laboratory-designated probe set algorithm. It serves as an adjunct to conventional chromosome studies in adult patients with T-ALL and evaluates specimens where chromosome studies are unsuccessful. Importantly, this test should not be used to screen for residual T-ALL.

Special Instructions

This test is performed only on samples from patients aged 31 years and older with T-cell acute lymphoblastic leukemia/lymphoma. If ordered for patients aged 30 years or younger, the test will be canceled and reordered as TALFP / Pediatric T-Lymphoblastic Leukemia/Lymphoma panel, FISH, Varies. For patients on a Children's Oncology Group (COG) protocol, the test will be canceled and reordered as COGTF. Avoid duplicate testing by following these guidelines.

Limitations

This test was developed according to CLIA requirements and is not FDA-approved. It should not replace conventional chromosome studies, which detect additional abnormalities associated with other hematologic disorders. FISH results may not detect all chromosomal abnormalities in T-ALL. Lack of FISH results can occur if no FISH signals are observed post-hybridization.

Test Details

New York Approved

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

No genes

Gene

JAK2 rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

ABL1 amplification or rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

KMT2A rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

STIL deletion • Other • Categorical (e.g., Positive / Negative / Indeterminate)

TLX3::BCL11B fusion • Fusion • Categorical (e.g., Positive / Negative / Indeterminate)

TRB rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

TRA rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

MLLT10::PICALM fusion • Fusion • Categorical (e.g., Positive / Negative / Indeterminate)

PDGFRB rearrangement • Other • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Order Codes

101663-3

Result Codes

50397-9
69965-2
93356-4
62356-1
42349-1
31208-2
85069-3
48767-8
62364-5
18771-6

Result Turnaround Time

7-10 days

Specimen

Bone Marrow

Volume

2 to 3 mL

Minimum Volume

1 mL

Container

Yellow top (ACD); Green top (sodium heparin) or lavender top (EDTA) acceptable

Collection Instructions

It is preferable to send the first aspirate from the bone marrow collection. Invert several times to mix bone marrow. Send bone marrow specimen in original tube. Do not aliquot.

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