T-Cell Receptor Gene Rearrangement, PCR, Bone Marrow

Casandra

Casandra Test Code MC32612Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID TCGBMCPT Codes 81340, 81342

T-Cell Receptor Gene Rearrangement, PCR, Bone Marrow

Clinical Use

Use

This test is used to determine whether a T-cell population is polyclonal or monoclonal using bone marrow specimens. It helps distinguish abnormal T-cell proliferations, which may suggest neoplastic processes, from reactive or benign T-cell expansions. Molecular techniques, including PCR, detect distributive differences in TCR gene sequences and genomic rearrangement fragment sizes, ultimately identifying the clonal nature of T-cell populations.

Special Instructions

Specimen must arrive within 7 days of collection. Relevant clinical information and cytogenetics results should be included if available.

Limitations

The detection of a clonal TCR-gene rearrangement does not necessarily indicate the presence of a T-cell neoplasm. False-positive results can occur due to the sensitivity of PCR and skewed amplification of target T-cell gene rearrangements. False negatives may arise from issues like tissue sampling or poor amplification. Interpretation should be contextual, considering clinicopathologic information, as it can sometimes be subjective.

Test Details

New York Approved