T-Cell Receptor Gene Rearrangement, PCR, Bone Marrow
Use
This test is used to determine whether a T-cell population is polyclonal or monoclonal using bone marrow specimens. It helps distinguish abnormal T-cell proliferations, which may suggest neoplastic processes, from reactive or benign T-cell expansions. Molecular techniques, including PCR, detect distributive differences in TCR gene sequences and genomic rearrangement fragment sizes, ultimately identifying the clonal nature of T-cell populations.
Special Instructions
Not provided.
Limitations
The detection of a clonal TCR-gene rearrangement does not necessarily indicate the presence of a T-cell neoplasm. False-positive results can occur due to the sensitivity of PCR and skewed amplification of target T-cell gene rearrangements. False negatives may arise from issues like tissue sampling or poor amplification. Interpretation should be contextual, considering clinicopathologic information, as it can sometimes be subjective.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
No genes
Gene
LOINC Codes
- 22637-3 - Path report.final Dx Spec
- 19139-5 - Pathologist name
Result Turnaround Time
5-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA) or Yellow top (ACD)
Collection Instructions
Invert several times to mix bone marrow. Send bone marrow specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis; moderately to severely clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |