T-Cell Receptor Gene Rearrangement, PCR, Varies
Use
This test is useful for determining whether a T-cell population is polyclonal or monoclonal using body fluid or tissue specimens. It identifies the presence of clonal T-cell receptor (TCR) gene rearrangements, which are markers of abnormal T-cell proliferations commonly seen in neoplastic conditions. The test can distinguish these clonal expansions from reactive, polyclonal T-cell populations, which are typical of non-neoplastic lymphoid proliferations.
Special Instructions
Not provided.
Limitations
The detection of clonal TCR-gene rearrangement does not necessarily indicate the presence of a T-cell neoplasm as false positives may occur due to the sensitivity of PCR. False-negative results can happen due to factors like poor amplification, or inadequate tissue sampling. The skewed amplification problem may arise when the total T-cell number in a sample is limited. Interpretation of presence or absence of predominant TCR-gene rearrangement profile is subjective and needs to be contextualized within other clinicopathologic information.
New York Approved
Methodology
PCR-based (Multiplex Primer)
Biomarkers
No genes
Gene
LOINC Codes
- 31208-2 - Specimen source
- 22637-3 - Path report.final Dx Spec
- 19139-5 - Pathologist name
Result Turnaround Time
7-14 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Paraffin block
Collection Instructions
Indicate specimen source and include pathology report. Decalcified specimens are not acceptable.
Causes for Rejection
Bone marrow core biopsies, Paraffin shavings
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient |
| Refrigerated | Yes |
| Frozen | Frozen |