Testosterone, Total and Bioavailable, Serum
Use
This test is recommended for assessing androgen status in cases with suspected sex hormone-binding globulin abnormalities, evaluating functional circulating testosterone in early pubertal boys, older men, and women exhibiting symptoms of hyperandrogenism with normal total testosterone levels. It is also used for monitoring testosterone or antiandrogen therapy. Testosterone, the primary androgenic hormone, is crucial for development of male sexual characteristics and has roles in both sexes such as estrogen precursor in females and anabolic effects across genders.
Special Instructions
Patient's age and sex are required. Use of oral contraceptives can affect testosterone and sex hormone-binding globulin levels, so it is crucial to be informed about the patient's medication status.
Limitations
Testosterone levels may fluctuate significantly between different days, leading to potential variability in results. Early morning collection is preferred as testosterone levels are typically higher then. The presence of oral contraceptives may reduce testosterone levels and increase sex hormone-binding globulin, affecting the free and bioavailable testosterone levels. The method's accuracy depends on proper handling and processing of the specimen - improper specimen collection or handling may interfere with results.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 58716-2
- 2990-0
- 2986-8
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.6 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 60 days |