Tobramycin, Peak, Serum
Use
The test is primarily used for monitoring the adequacy of serum concentration during tobramycin therapy. Tobramycin is an antibiotic effective against life-threatening blood infections caused by gram-negative bacilli, including organisms such as Citrobacter freundii, Enterobacter, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, and Serratia species. Monitoring is critical as toxicities can occur, like ototoxicity and nephrotoxicity, particularly when used concurrently with other similar risk drugs.
Special Instructions
Sample collection must adhere to guidelines ensuring serum gel tubes are centrifuged within 2 hours. If red-top tubes are used, the serum should be aliquoted into a plastic vial within 2 hours post-collection. The accompanying form 'Therapeutics Test Request' should be completed and sent with the specimen if not ordered electronically.
Limitations
The test focuses on measuring the serum concentration of tobramycin and does not replace clinical assessments for ototoxicity and nephrotoxicity risks. The presence of similar enzyme immunoassays should be ensured for accurate competition for antibody binding sites. Potential interference by endogenous substances is minimized by utilizing bacterial enzyme activity in assays.
FDA ApprovedNew York Approved
Methodology
Immunoassay
Biomarkers
LOINC Codes
- 4057-6
- 4057-6
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.25 mL
Container
Plastic vial
Collection Instructions
Serum gel tubes must be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged and the serum aliquoted within 2 hours.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 7 days |
| Frozen | 28 days |