Tropheryma whipplei, Molecular Detection, PCR, Varies
Use
This test aids in diagnosing Whipple disease, particularly in identifying inconclusive or suspicious cases using tissue or fluid specimens. Whipple disease is a chronic, systemic illness primarily affecting the small intestine and lymphatic drainage, which can lead to malabsorption, chronic diarrhea, abdominal pain, arthralgia, fever, and central nervous system symptoms. The disease often requires a histologic examination of biopsy specimens from involved tissues for diagnosis.
Special Instructions
Not provided.
Limitations
A negative result does not negate the presence of the organism, as it may occur due to inhibition of polymerase chain reaction, sequence variability underlying primers or probes, or presence of Tropheryma whipplei DNA below the assay's detection limit. The test should be used as an adjunct in diagnosis and not as the sole criteria for clinical decision-making.
New York Approved
Methodology
PCR-based (Real-Time Polymerase Chain Reaction)
Biomarkers
LOINC Codes
- 97206-7 - T whipplei DNA Spec Ql NAA+n-prb
- 31208-2 - Specimen source
- 97206-7 - T whipplei DNA Spec Ql NAA+n-prb
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Entire collection or 5 mm(3)
Minimum Volume
5 mm(3)
Container
Sterile container
Collection Instructions
Collect fresh tissue specimen. Submit tissue only, do not add fluid to tissue. Refrigerate or freeze specimen.
Storage Instructions
Refrigerated (preferred) 7 days /Frozen 7 days
Causes for Rejection
Tissue in formalin, formaldehyde, or acetone; specimens other than those listed; slides
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |