Tryptase Immunostain, Technical Component Only

Casandra

Casandra Test Code MC69555Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID TRPTSCPT Codes 88341, 88342

Tryptase Immunostain, Technical Component Only

Clinical Use

Use

This test is useful as a marker of mast cells. It supports the diagnosis of mast cell disease due to the characteristic immunohistochemical staining pattern the tryptase antibody produces in mast cells. In patients with suspected mast cell disorders, the staining provides important diagnostic information.

Special Instructions

This test includes only the technical performance of the stain and does not include pathologist interpretation. Technical component only stains should not be ordered with PATHC/Pathology Consultation as they will be canceled if done so. For a complete diagnostic evaluation, order PATHC for a review by a qualified pathologist.

Limitations

The test is limited by the age of cut paraffin sections, which can affect immunoreactivity. Stability thresholds vary among published literature, and antigen stability is time-dependent, suggesting that paraffin sections should ideally be cut within 6 weeks to avoid diminished staining quality. Environmental factors affecting the charge of glass slides can also alter staining consistency, with improper storage leading to inconsistent results.

Test Details

FDA Approved

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

Tryptase

Protein

IHC • Expression • Ordinal Score (e.g., TPS, CPS, H-score)

LOINC Codes

Order Codes

Order only;no result

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Immunostain Technical Only Envelope

Collection Instructions

Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the transport container.