Ureaplasma species, Molecular Detection, PCR, Blood
Use
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from whole blood. This test is not intended for medicolegal use. A positive PCR result for the ureC gene indicates presence of U urealyticum or U parvum DNA; a negative result suggests absence but does not rule out infection due to possible PCR inhibition, sequence variability, or low quantity below detection limit. Results should be interpreted along with clinical context, acknowledging Ureaplasma may be part of normal genital microbiota.
Special Instructions
The assay employs Real‑Time PCR using LightCycler and FRET hybridization probes targeting the ureC gene, differentiating U urealyticum and U parvum in a closed-tube system. The high sensitivity demands processing in a contamination-free environment.
Limitations
False-negative results may occur due to PCR inhibition, sequence variability in primers or probes, or organism loads below detection threshold. The assay does not detect other Mycoplasma or Ureaplasma species, including Mycoplasma pneumoniae. Because Ureaplasma species can be part of normal genital microbiota, clinical interpretation must consider context.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA) preferred; acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Collection Instructions
Send whole blood specimen in original tube. Process in environment minimizing contamination by Ureaplasma DNA.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |