Ureaplasma species, Molecular Detection, PCR, Plasma
Use
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from plasma. A positive real‑time PCR result targeting the ureC gene indicates presence of U urealyticum or U parvum DNA. Negative result does not rule out infection due to possible PCR inhibition, primer/probe sequence variability, or low organism burden. Since Ureaplasma species may be part of normal microbiota, results should be interpreted in clinical context. It is not intended for medicolegal use.
Special Instructions
This assay employs real‑time PCR with fluorescence resonance energy transfer (FRET) hybridization probes on the LightCycler platform targeting the ureC gene; processing must limit potential contamination by Ureaplasma DNA. NY State approval: Yes. Performing lab: Mayo Clinic Laboratories, Rochester Main Campus.
Limitations
False negatives may occur due to PCR inhibition, sequence variability underlying primers/probes, or low organism quantities. It does not detect other Ureaplasma or Mycoplasma species (including M pneumoniae). Interfering substances and specimens contaminated by DNA can affect accuracy. Interpretation requires clinical and epidemiological correlation. Not for medicolegal use.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 69934-8
- 31208-2
- 51988-4
- 69933-0
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA) preferred; acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA‑derived aliquot; submission in screw‑capped sterile container
Collection Instructions
Centrifuge and aliquot plasma into a plastic vial
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |