Ureaplasma species, Molecular Detection, PCR, Varies
Use
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from genitourinary, reproductive, bone, spine, joint, and lower respiratory sources. Ureaplasma urealyticum and Ureaplasma parvum have been associated with clinically significant infections, including urethritis, epididymitis, upper urinary tract infections, and infections in neonates, such as meningoencephalitis and pneumonia. They have also been detected in amniotic fluid of women with preterm labor and can cause hyperammonemia in lung transplant recipients. PCR allows for rapid detection and differentiation from other cultures, which require significant technical skill and time.
Special Instructions
Test is not intended for medicolegal use. Submit only one of the following specimens: Swab, Fluid, Respiratory, Synovial fluid, Urine-first void, Tissue. Ensure the specimen is processed in an environment to minimize contamination by Ureaplasma DNA. Minimum volume for urine-first void is 2 mL.
Limitations
A negative PCR result does not rule out infection, as false-negative results may occur due to inhibition of PCR, sequence variability, or low quantities of U urealyticum or U parvum. Interfering substances may affect accuracy; results should be interpreted with clinical and epidemiological findings. Ureaplasma species are part of the normal microbiota and results should be considered accordingly.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 69934-8
- 31208-2
- 69933-0
- 51988-4
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Buccal Swab
Volume
1 Swab
Minimum Volume
Not provided
Container
Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium); Acceptable: Swab in transport media: M4, M4-RT, M5, M6, universal transport media, or ESwab
Collection Instructions
Vaginal: Collect specimen by swabbing back and forth over mucosa surface. Urethra or cervical: Insert swab 1 to 3 cm and rotate 360 degrees. Wound: Swab back and forth over wound surface.
Causes for Rejection
Cotton or calcium alginate-tipped swab, wood shaft swab, transport swab with gel or charcoal, formalin-fixed tissues, Port-a-Cul tube, anaerobic fluid vials, dry swab, bone marrow, decalcified bone, slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |