Varicella-Zoster Virus, Molecular Detection, PCR, Varies
Use
This test is useful for the rapid (qualitative) detection of varicella-zoster virus DNA in clinical specimens for laboratory diagnosis of diseases caused by this virus, such as chickenpox (varicella) and shingles (herpes zoster). Detection of varicella-zoster virus (VZV) DNA supports the clinical diagnosis of infection due to this virus. VZV DNA is not detected in cerebrospinal fluid from patients without central nervous system disease caused by this virus. The test is performed using real-time PCR/DNA probe hybridization method, which enables rapid detection with high specificity.
Special Instructions
The test should not be used to screen asymptomatic patients. Specimen source is required, and source information must include the main anatomical site of collection. If not ordering electronically, complete, print, and send a Microbiology Test Request form (T244) with the specimen.
Limitations
This test is a qualitative assay and results are reported as negative or positive for target VZV DNA. A negative result does not exclude the possibility of varicella-zoster virus (VZV) infection. The assay should only be used for patients with a history and symptoms consistent with VZV infection. The reference range is typically "negative" for this assay, and it must be interpreted in the context of the clinical picture. The assay has a limit of detection of 10 to 20 DNA target copies per microliter, and there is no PCR signal from extracts of 27 bacterial, viral, and fungal isolates that could cause similar symptoms.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 94584-0
- 94584-0
- 31208-2
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile screw cap vial
Collection Instructions
Do not centrifuge.