West Nile Virus, RNA, PCR, Molecular Detection, Blood
Use
Rapid testing for West Nile virus (WNV) RNA (lineage 1 and lineage 2). An adjunctive test to serology for detection of early WNV infection (ie, first few days after symptom onset), with blood specimens potentially providing a greater sensitivity than other sources for WNV RNA detection. Not intended for screening asymptomatic individuals; use only in patients with signs and symptoms of WNV disease.
Special Instructions
Not provided.
Limitations
Sensitivity is highly dependent on timing relative to illness onset; greatest utility is during the first few days of symptom onset. A negative result does not exclude WNV infection—results should be interpreted alongside clinical findings and serologic testing. The assay detects both viable and nonviable virus, and performance depends on viral load, which may not correlate with cell culture.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 34460-6 - WNV RNA Bld Ql NAA+probe
- 34460-6 - WNV RNA Bld Ql NAA+probe
Result Turnaround Time
0-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Not provided
Minimum Volume
Not provided
Container
EDTA (lavender‑top) tube
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |