Zika Virus, PCR, Molecular Detection, Serum
Use
This test provides qualitative detection of Zika virus RNA in serum from individuals meeting the CDC Zika virus clinical or epidemiologic criteria. It is particularly useful for evaluating pregnant women and symptomatic nonpregnant individuals with potential exposure to the Zika virus. The test can identify the presence of the virus during the acute phase of infection, which is critical for managing potential health risks associated with Zika virus exposure.
Special Instructions
It is recommended to perform concurrent testing for RNA or IgM-class antibodies to dengue and chikungunya viruses, due to similar clinical presentations with Zika virus infection. The test should be ordered alongside a Microbiology Test Request Form if not placed electronically.
Limitations
Negative results do not rule out the possibility of Zika virus infection. False-negative results may occur if the specimen degrades during transit or if collected outside the time Zika virus RNA is typically present post-symptom onset. All results should be interpreted by professionals, considering the patient's clinical signs and history. Additionally, Zika virus testing is not advised for asymptomatic couples trying to conceive due to potential false results and the persistence of the virus in reproductive fluids.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 85622-9
- 85622-9
- 69048-7
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.
Causes for Rejection
Gross hemolysis, Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |