20q12, 20q13.3 Deletion
Use
The FISH test is performed on hematologic specimens to detect the 20q deletion, which is commonly observed in myeloid neoplasms. This deletion is associated with conditions such as acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and myeloproliferative disorder (MPD). Detecting this chromosomal anomaly aids in the diagnosis and management of these hematological malignancies.
Special Instructions
All attempts will be made to process and report samples received more than 72 hours post-collection. Bone marrow is the sample of choice in most hematologic malignancies due to its significance in diagnosis. Specimens must be handled and shipped under specified conditions to ensure sample integrity.
Limitations
The test is designed specifically to detect deletions at the 20q12 and 20q13.3 loci using FISH methodology. It may not detect other genetic alterations outside these specified regions. The assay requires viable cells; thus, clotted specimens or those exposed to extreme temperatures may result in test rejection. The test is analytical and interpretive performance may vary based on specimen quality and handling.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
Not provided
Container
EDTA preferred (NaHep accepted)
Storage Instructions
2°C to 25°C
Causes for Rejection
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |