API2 (BIRC3)::MALT1; t(11;18)", paraffin
Use
FISH testing on paraffin-embedded cells for detection of API2::MALT1 gene fusion. This fusion, associated with t(11;18), is frequently observed in MALT lymphomas (also known as extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue) and constitutes about 8% of all non-Hodgkin lymphomas. The most common structural abnormality associated with MALT lymphomas is the translocation between API2 and MALT1.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Limitations
The test is performed by an affiliate laboratory. It is important that the tissues are correctly fixed as specified since improper fixative or decalcification can render the specimen unsuitable for FISH analysis. Extreme temperatures during shipping can also impact specimen quality.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue Block
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Storage Instructions
Store at 15°C to 25°C
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |