BCR/ABL1 qRT PCR, minor
Use
This test uses quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for the detection of the minor BCR::ABL fusion gene transcript p190 (e1a2) in patients with Philadelphia chromosome (t(9;22)(q34;q11.2)) positive leukemia, commonly associated with Acute Lymphoblastic Leukemia (ALL). It aids in monitoring disease progression and response to treatment.
Special Instructions
Frozen blood samples are NOT accepted. Notify MPLN if shipment is to arrive after 1 p.m. Friday or Saturday.
Limitations
Specimen stability requires receipt by MPLN within 48 hours of collection. Incorrect specimen handling, such as clotted samples, incorrect anticoagulants, or storage outside recommended temperatures, can lead to specimen rejection. Isolated RNA of suboptimal quantity and/or quality may affect test results.
Methodology
PCR-based (qPCR)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
5 mL
Container
EDTA preferred (NaHep accepted)
Storage Instructions
2-8°C
Causes for Rejection
Specimen >48 hours old; Specimen clotted; Specimen stored or shipped at incorrect temperature; Specimen in incorrect anticoagulant; Insufficient specimen volume; Isolated RNA of suboptimal quantity and/or quality
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 48 hours |