Bronchoalveolar Lavage
Use
Flow cytometry panel inclusive of CD3, CD4, CD8, and CD16 in bronchoalveolar lavage specimens. Reporting includes CD4:CD8 ratio and natural killer (NK)-cell enumeration. This test is associated with pulmonary diseases, sarcoidosis, and lung transplantation, providing significant insights into immune cell populations in bronchoalveolar lavage fluid.
Special Instructions
Expected turnaround time is 1 day, but this can vary depending on biopharma protocol requirements. Ensure that specimens are stored and shipped under recommended conditions to prevent rejection. Use Oncology Pathology Requisition form for test orders.
Limitations
Specimen stability is 48 hours at 4°C, which must be accounted for during shipping. Potential causes for rejection include incorrect temperature storage, non-viable specimens, insufficient cell counts, hemolysis, or clotting.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
10 mL
Minimum Volume
5 mL
Container
Sterile container with 2-4 mL MPLN RPMI transport media
Storage Instructions
Store at 2°C to 8°C
Causes for Rejection
Specimens stored at incorrect temperature; Non-viable specimens; Too few cells; Hemolysis; Specimen clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 48 hours at 4°C |