Caldesmon
Use
Caldesmon is a regulatory protein found in smooth muscle. This IHC antibody is useful in labeling smooth muscle and tumors of smooth muscle, myofibroblast, and myoepithelial differentiation. It is also used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary.
Special Instructions
For fresh tissues, samples may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is not preferred. Samples must be tested 3 months after placing in ethanol. Unstained slides should be left unbaked and should be on positively charged glass slides to prevent background staining and tissue loss.
Limitations
Stated turnaround times are subject to change based on biopharma protocol requirements. Other fixatives apart from 10% NBF or B plus lead to specimen rejection. Samples must be tested within the specified time frames based on fixation methods to ensure accurate results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred). Use a refrigerated gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Storage Instructions
15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |