Caldesmon, technical only
Use
Caldesmon is a regulatory protein found in smooth muscle. This IHC antibody is useful in labeling smooth muscle and tumors of smooth muscle, myofibroblast, and myoepithelial differentiation. Further, caldesmon is also used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. Technical component only. Pathologist read not included.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Test is technical component only; no pathology interpretation is included. Specimens fixed with other than specified fixatives will be rejected. Fresh tissues have a limited stability of up to 72 hours. Specimens in ethanol must be tested within 3 months. Use of unstained slides has indefinite stability but requires positively charged glass slides.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
0 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Use a fixative to tissue ratio of 20:1 with 10% neutral buffered formalin for 6-72 hours.
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated gel pack in the shipment to protect from extreme temperatures. Separate from specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |