Calretinin
Use
Calretinin is a calcium binding protein used to differentiate mesothelioma (positive) from carcinoma (negative) and schwannoma (positive) from neurofibroma (negative). It is also employed in diagnosing Hirschsprung disease, aiding in the identification of specific pathological conditions based on differential staining patterns.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested within 3 months after placing in ethanol. Positively charged glass slides are required to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% neutral buffered formalin (NBF) or B plus. All other fixatives will be rejected. The test is subject to potential variation based on differing fixation times and methods which can impact the immunohistochemical staining results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Use a refrigerated (NOT FROZEN) gel pack in shipment to protect from extreme temperatures. Separate gel pack from specimen.
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | Indefinite |
| Frozen | Indefinite |