Cathepsin D
Use
Cathepsin D is an aspartyl protease involved in protein catabolism and tissue remodeling, secreted from cancer cells. Elevated serum levels of cathepsin D are associated with poor prognosis in glioma patients, indicating its potential as a serum marker for aggressive gliomas. Autoantibodies against procathepsin D are identified as potential diagnostic and predictive markers for various cancers.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. This is NOT preferred. Samples must be tested within 3 months after placing in ethanol. Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% neutral buffered formalin or B plus; all other fixatives will be rejected. Fresh tissues should be fixed for 6-72 hours, preferably 48-72 hours.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C; avoid freezing or exposure to extreme temperatures by using a refrigerated gel pack.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |