Cathepsin D, technical only
Use
Cathepsin D is an aspartyl protease involved in protein catabolism and tissue remodeling, which can be secreted from cancer cells. Elevated serum levels of cathepsin D have been associated with poor prognosis in glioma patients, suggesting its potential as a serum marker for aggressive gliomas. Additionally, autoantibodies against procathepsin D have been identified as potential diagnostic and predictive markers for various cancers. This test focuses on the technical component only, without pathologist reading.
Special Instructions
The test requires fresh tissues and uses specific fixative protocols. It's important to use positively charged glass slides for unstained slides to prevent background staining and tissue loss.
Limitations
This test does not include a pathologist read. Specimens fixed in fixatives other than 10% NBF or B plus will be rejected. Specimens must comply with specific fixation and storage guidelines, particularly those transferred to 70% ethanol.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |