CD123
Use
CD123 serves as a diagnostic, prognostic, and therapeutic marker in some hematologic malignancies, particularly acute leukemia. Its expression is highly specific and sensitive for identifying blastic plasmacytoid dendritic cell neoplasm when co-expressed with TCF4. It is associated with high marrow blast percentages, monocytic differentiation, and CD34 negativity, indicating its significant role in leukemic maintenance and creation.
Special Instructions
Fresh tissues can be fixed in formalin for 24-72 hours by collection sites and transferred to 70% ethanol for long-term storage at 2-8°C. Samples must be tested within 3 months after being placed in ethanol. Unstained slides require positively charged glass slides to avoid background staining and tissue loss and should remain unbaked.
Limitations
The immunohistochemistry method is exclusive to the detection of CD123 antigen on tissues. Specimens not fixed in 10% neutral buffered formalin or B plus can be rejected. Highly dependent on specimen handling, and fixation time. Interpretation can be subjective and requires a qualified pathologist.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Stored at 15°C to 25°C. Specimens can be transferred to 70% ethanol.
Causes for Rejection
Rejected if not fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | Indefinite |
| Frozen | Indefinite |