CD15
Use
CD15 antigen is reported to be expressed in human granulocytes (~90%) and circulating monocytes (~30-60%) while being absent on normal lymphocytes. CD15 is also expressed on Reed Sternberg cells of Hodgkin's disease and some leukemias. This test is useful for diagnosing and characterizing hematological malignancies, particularly Hodgkin's disease and certain leukemias, by detecting CD15 expression.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Unstained slides require positively charged glass slides to prevent background staining and tissue loss, and should be left unbaked.
Limitations
The test is limited to detecting the presence of CD15 expression in tissue samples. Results interpretation may be affected by the quality of the sample fixation and slide preparation. Other fixatives apart from 10% NBF or B plus may lead to specimen rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store between 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |