CD15, technical only
Use
CD15 antigen is reported to be expressed in human granulocytes (~90%) and circulating monocytes (~30-60%) while being absent on normal lymphocytes. CD15 is also expressed on Reed Sternberg cells of Hodgkin's disease and some leukemias. This test is used to detect the presence of CD15 antigen, particularly in technical settings where a pathologist review is not included.
Special Instructions
Not provided.
Limitations
This is a technical component only and does not include a pathologist read. Fixatives other than 10% NBF or B plus are not accepted, and inappropriate fixation methods can lead to specimen rejection. Specimens must be analyzed within specified stability windows to ensure reliability. The test is primarily used for detecting CD15 antigen expression and cannot be used for other diagnostic purposes without further analysis.
Methodology
Immunoassay (IHC)
Biomarkers
CD15
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C; transfer to 70% alcohol for long-term storage after initial fixation if needed.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |