CD163, technical only
Use
CD163 is a type I membrane protein restricted in expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells, and Langerhans cells. This test is technical only and does not include a pathologist read.
Special Instructions
Samples must be tested within 3 months after placing in ethanol. Positively charged glass slides are required to prevent background staining and prevent tissue loss. Slides should be left unbaked to maintain integrity during transport.
Limitations
This test evaluates only the technical component, and a pathologist read is not included. Specimens must be fixed in 10% neutral buffered formalin or B plus, as all other fixatives will be rejected. Specimen integrity must be maintained by adhering to recommended storage temperatures and stabilization post-collection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
3-5 μM Formally Fixed Paraffin Embedded Tissue slide
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated gel pack to protect from extreme temperatures during shipment. Do not freeze.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Refrigerated | Indefinite |
| Frozen | Indefinite |