CD19
Use
CD19 is a member of the immunoglobulin superfamily expressed on the surface of B lymphocytes and follicular dendritic cells. It's a critical regulator in B-cell development, activation, and differentiation, expressed from the earliest recognizable B-cell lineage stage throughout development, and then lost upon plasma cell maturation.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Samples must be tested within 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
The clinical interpretation of CD19 depends on the care taken during pre-analytical processes such as proper fixation and slide preparation. Improper storage can lead to inaccurate results due to loss of antigenicity.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Unknown)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). FFPE Block: Formalin Fixed. Unstained slides: 2 slides (3-5 μM) per stain.
Storage Instructions
15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Fresh Tissue: ≤72 Hours. FFPE blocks: Indefinite. Unstained slides: Indefinite. |