CD33, technical only
Use
CD33 antigen is expressed on myelomonocytic precursor cells and throughout myeloid and monocyte maturation, but is absent on granulocytes. Some acute undifferentiated leukemias and acute lymphoblastic leukemias have been reported to express CD33.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Positively charged glass slides are required to prevent background staining and prevent tissue loss.
Limitations
Technical component only. Pathologist read not included. Specimens fixed in fixatives other than 10% NBF or B plus will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% NBF for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |