CD42b, technical only
Use
CD42b glycoprotein is expressed on the cell surface of megakaryocytes in bone marrow and platelets in blood vessels. Occasional staining is also observed in the basal epithelial of skin. Absence of CD42b antigen on platelets is reported to be a possible indicator of Bernard-Soulier disease. Technical component only.
Special Instructions
Fresh tissues should be fixed at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours. Positively charged glass slides are required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
This test is the technical component only and does not include a pathologist read. Stained slides have indefinite stability, but fresh tissues must be tested within 72 hours and can be stored in 70% alcohol for transportation. Only specimens fixed in 10% NBF or B plus are acceptable; others will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C. Use refrigerated gel pack in shipment to avoid freezing.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |