CD45
Use
CD45, also known as leukocyte common antigen, is a glycoprotein that is ubiquitously expressed on the cell surface of nucleated hematopoietic cells, including lymphocytes, monocytes, and eosinophils. It plays a key role in the regulation and activation of the immune system, being essential for proper immune function. It is notably absent from erythrocytes and platelets, making it a critical marker in differentiating cell types in hematopoietic studies.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours by collection sites and then transferred to 70% ethanol for longer term storage if necessary. Unstained slides require positively charged glass to prevent background staining and tissue loss. Unbaked slides are required to maintain quality.
Limitations
Specimens must be fixed in 10% neutral buffered formalin to avoid rejection. Alternative fixatives are not accepted, which can limit the acceptance of certain specimen preparations. Tissue fixation beyond preferred time frames may impact results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Storage Instructions
Store at 15°C to 25°C. For longer-term storage, specimens transferred to 70% ethanol should be stored at 2-8°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |