CD45, technical only
Use
CD45 (leukocyte common antigen) is a glycoprotein ubiquitously expressed on the cell surface of nucleated hematopoietic cells (including lymphocytes, monocytes, and eosinophils), but is absent from erythrocytes and platelets. Expression of CD45 is necessary in the regulation and activation of the immune system. The test is technical only and does not include a pathologist read.
Special Instructions
Not provided.
Limitations
The test does not include a pathologist interpretation. CD45 expression may vary based on cell type, state of differentiation, or activation status. Unstained slides must be unbaked to avoid interference with staining quality. Fixation conditions play a critical role and must be adhered to for accurate results.
FDA Approved
Methodology
Immunoassay (IHC)
Biomarkers
CD45
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures; separate gel pack from specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |