CD5
Use
CD5 is a transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes, and some B lymphocytes (B1a). CD5 can be used as an aid in the diagnosis of lymphomas and thymic carcinoma as well as differentiating chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) from other types of low grade B-cell non-Hodgkin lymphomas.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. For unstained slides, positively charged glass slides are required to prevent background staining and tissue loss, and slides should be left unbaked.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract. Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C, use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Frozen | Indefinite |