CD57, technical only
Use
CD57 glycoprotein is found on a subset of natural killer (NK) cells, large granular lymphocytes, and on neuroectodermal cells expressing myelin-associated glycoproteins. Cells co-expressing CD57 and CD8 play a role in the rejection of grafts in acute graft vs host disease. CD57 staining is also observed in neuroendocrine tumors. Technical component only. Pathologist read not included.
Special Instructions
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. The test does not include a pathologist read and is for the technical component only. Turnaround time may vary based on biopharma protocol requirements. Specimen stability for fresh tissues is limited to 72 hours, while FFPE blocks and unstained slides are indefinite but must meet specified conditions.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |