CD99
Use
CD99 is implicated in cellular processes such as homotypic aggregation of T-cells and apoptosis of thymocytes. It is expressed on most human tissues and hematopoietic cell types, aiding in diagnosing tumors like Ewings sarcoma and T-cell lymphoma.
Special Instructions
Ensure specimens are well-fixed using 10% neutral buffered formalin. Positively charged glass slides are required to prevent background staining.
Limitations
Only specimens fixed in 10% NBF or B plus are acceptable. Other fixatives will lead to rejection. Slides must remain unbaked and require proper fixation duration for accurate results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Use a fixative to tissue ratio of 20:1. Fix in 10% NBF for 6-72 hours.
Storage Instructions
Store at 15°C to 25°C. May transfer to 70% ethanol for long-term storage.
Causes for Rejection
Fixation with non-approved fixatives.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |