Chromogranin A, technical only
Use
Chromogranin A is a glycoprotein found in neuroendocrine cells. In pathology, Chromogranin A is used to diagnose and monitor various neuroendocrine malignancies, such as pheochromocytomas, small cell lung carcinoma, and gastrointestinal carcinoids, where its elevated levels indicate neuroendocrine differentiation.
Special Instructions
Technical component only. Pathologist read not included. Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Stated turn-around-times are for clinical use only and subject to change based on biopharma protocol requirements. Specimens must be tested within 3 months after placing in ethanol.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Specimens may be fixed in 10% NBF or B plus and transferred to 70% alcohol for transportation after fixation.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Refrigerated | Indefinite |
| Frozen | Indefinite |