CK 19
Use
Cytokeratin 19 (CK19) is a low-molecular-weight keratin protein expressed in simple epithelia, particularly in biliary and ductal cells, and is commonly found in a variety of carcinomas. In pathology, CK19 is used to identify and diagnose carcinomas, particularly those of epithelial origin such as cholangiocarcinoma, pancreatic cancer, and breast cancer.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Stated turnaround times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. All other fixatives than 10% NBF or B plus will be rejected. Fresh tissues must be fixed for 6-72 hours, preferably 48-72 hours.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Ambient, Specimens may be transferred to 70% alcohol for transportation purposes after fixation
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |